Were there any gags used in the live-action wraparounds on The New 3 Stooges 1965 series which were NEVER used in any of their Columbia shorts?
Yes. Among them...
"Melodrama" (aka "Pay the Rent") - A Healy routine performed in PLANE NUTS (1933 MGM). The Stooges did it in the feature TIME OUT FOR RHYTHM (1941), but not in any of the shorts.
"Barbers" - A variation of a routine performed in SCREEN SNAPSHOTS Series 14 # 6
, "You have to belong to the union to do that..."
"Magicians" - A old routine, new to the Stooges.
Others. Ed Bernds wrote all those wrap-arounds (not Lee Orgel, as he once claimed in an interview), and recycled many old gags, but they were new material for the onscreen Stooges.